Composite

Part:BBa_K4160011:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


GEMS receptor construct containing PR3 as affinity domain with 31 amino acid linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2414
    Illegal PstI site found at 191
    Illegal PstI site found at 1102
    Illegal PstI site found at 2097
    Illegal PstI site found at 2258
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 733
    Illegal NheI site found at 1471
    Illegal PstI site found at 191
    Illegal PstI site found at 1102
    Illegal PstI site found at 2097
    Illegal PstI site found at 2258
    Illegal NotI site found at 2401
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 733
    Illegal BglII site found at 1575
    Illegal BglII site found at 1761
    Illegal BglII site found at 2025
    Illegal BamHI site found at 64
    Illegal XhoI site found at 985
    Illegal XhoI site found at 2408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2414
    Illegal PstI site found at 191
    Illegal PstI site found at 1102
    Illegal PstI site found at 2097
    Illegal PstI site found at 2258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2414
    Illegal PstI site found at 191
    Illegal PstI site found at 1102
    Illegal PstI site found at 2097
    Illegal PstI site found at 2258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K4160004, and BBa_K2217015.

References